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The present use of these therapies remains restricted in human reproductive medicine because of both ethical and safety factors (223)
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After washing with PBS-T five times, secondary antibody-incubated membranes were imaged with the ChemiDocMP Imaging System (BIO-RAD Laboratories) directly with the IRDye800CW channel for visualisation of vinculin bands, or with the addition of western blotting detection reagent (SuperSignal™ West Dura Extended Duration Substrate, Thermo Fisher Scientific, #34075) for the HRP conjugated system with chemiluminescence channel
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