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The cocultures were either spotted onto a 0.22 μm nitrocellulose membrane (Nalgene) placed on YLB agar plates at 26 °C for 48 h (for contact-dependent competition), or inoculated into 2 ml YLB medium at 26 °C with shaking for 24 h or 48 h (for contact-independent competition in liquid medium)

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Further studies are needed to determine whether other factors may also be involved