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Flow cytometry We used an LSR II flow cytometer (Beckon Dickinson, San Jose, CA, USA) to detect platelet activation through several measurements: CD62p expression, the binding of PAC-1 antibody (BD Biosciences), which recognizes the active conformation of the fibrinogen receptor αIIbβ3, annexin V binding,9 platelet-bound fibrinogen and coagulation factor V,2625 and the formation of platelet-leukocyte complexes ( Online Supplementary Methods )