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Synergy was defined as a ≥2 log10 CFU/mL reduction at 24 h in a combination when compared to the CFU/mL of the most active individual drug ( 2.5 Total ROS assay 2′,7′-dichlorofluorescein diacetate (DCFH-DA) dye was used to measure reactive oxygen species (ROS) levels in CRKP as previously described ( 2.6 Bacterial metabolite extraction Metabolite extraction was performed as described previously with a few modifications ( 2.7 LC‒MS/MS analysis and data preprocessing A UHPLC system (Vanquish, Thermo Fisher Scientific) with a UPLC HSS T3 column coupled to an Orbitrap Exploris 120 mass spectrometer (Orbitrap MS, Thermo) was employed to perform LC‒MS/MS analysis
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