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In vitro binding assay 3 pmol factor-free 80S with 24 pmol GST-NMT1 and 24 pmol NAC-wt, -mutants or individual components were incubated in Binding Buffer (20 mM HEPES/KOH pH 7.5, 100 mM KOAc, 5 mM MgCl 2 , 2 mM DTT) for 1 h on ice
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