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The Ig were eluted from the matrix into 12 µL phosphate neutralization buffer (429.3 mM sodium dihydrogen phosphate monohydrate, 470.7 disodium hydrogen phosphate dihydrate, pH 7.54) in low-bind Eppendorf tubes by adding 20 µL 0.1 M glycine (pH 2.7) onto the CaptureSelect affinity matrices
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