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Methotrexate exacerbates NAFLD by inhibiting antioxidant activity, boosting lipid buildup, and generating mitochondrial dysfunction, which leads to increased hepatocyte apoptosis and inflammation

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Inhibition of mTOR with rapamycin or its derivatives typically activates downstream factors such as HIF-1α and GLUT1, enhancing glucose uptake and glycolysis (143, 144)