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Hydrogen peroxide (H 2 O ₂ ) is needed as a substrate for this process

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Phosphorylation of eIF2α by GCN2 inhibits general cap-dependent protein synthesis via the ISR but induces cap-independent synthesis of the activating transcription factor 4 (ATF4), which in turn induces a pathway to increase transcription of ER-associated chaperones, halt cap-dependent translation, and eventually result in cell death 96