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High density lipoprotein cholesterol (HDL-C), mainly synthesized in the liver, is an anti-atherosclerotic lipoprotein that transports cholesterol from extrahepatic tissues to the liver for metabolism, and it is excreted from the body by bile (Deng et al., 2024)

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After incubation, the suspension was centrifuged at 2000 rpm for 5 min, and the supernatant was measured at 540 nm using a microplate reader