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The resulting homogenate was centrifuged (two min max rpm) and 600 µl supernatant was applied to an activated and equilibrated RP18 solid phase extraction column (activation with 1 ml acetonitrile and equilibration with 1 ml methanol/H 2 O [80/20, vol/vol] [Phenomenex Strata C18-E, 55 µm, 50 mg/1 ml
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