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For CUT&RUN, we selected antibodies against histone modification marks: H3 lysine 4 trimethylation (H3K4Me3), H3 lysine 27 acetylation (H3K27Ac) and H3 lysine 27 trimethylation (H3K27Me3), which are associated with active promoters, active promoter/enhancers and polycomb repression, respectively 20
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