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2.7 Immunohistochemistry Whole lenses collected at 10am (ZT4), 2pm (ZT8), 6pm (ZT12) and 10pm (ZT16) (n= 4 lenses for each time point) were fixed in 0.75% wt/vol paraformaldehyde, cryoprotected, and cryosectioned in an axial orientation using standard protocols developed in our laboratory