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Trypan blue for cell death and DAB staining for H 2 O 2 accumulation Detached leaves were immersed in trypan blue staining solution (2.5 mg ml −1 trypan blue dissolved in lactophenol:lactic acid:glycerol:liquid phenol:ddH 2 O at 1:1:1:1:1) for 30 min
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