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Following the culture, 20 oocytes per treatment were fixed in ethanol: acetic acid (3:1), stained with aceto-orcein (1%w/v), and observed under a phase contrast microscope

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The simultaneous development of multiple follicles in PCOS patients requires energy, and endocrine disorders (such as insulin resistance, elevated androgen hormones, etc.) are accompanied by abnormal glucose and lipid metabolism