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Knockdown of TMEM19 by siRNA results in failure to respond to the erucamide-stimulated paracrine effect of growth factors, which is consistent with in vivo knockdown of TMEM19 and inhibition of erucamide–TMEM19 downstream pathways by neutralizing antibodies (Fig
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119 Conversely, at the N-terminal domain, a non-catalytic regulatory haem sensor negatively impacts the enzymatic activity of CBS upon reduction and completely inactivates the enzyme upon binding of nitric oxide or carbon monoxide
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