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For example, p53 stabilization results in down-regulation of numerous metabolic genes, and we observe that overexpression of RNR subunits alone is not sufficient to re-sensitize arrested cells to ferroptosis, perhaps due to the absence of upstream precursors that would allow RNR to operate at full capacity
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Briefly, HUVECs (5.0×103 cells/cm2) in collagen-coated 24-well culture plates were treated with the abovementioned tube-induction medium containing EBC-1 supernatants (2–50 µg/ml) at the indicated time points
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