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This is often due to the use of vacuum filtration devices during sterilization, which reduces the concentration of HCO 3 - ions in the culture medium and consequently raises the pH value

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A decrease in mean arterial blood pressure (MABP) can compromise cerebral perfusion pressure (CPP) and cerebral blood flow (CBF), potentially exacerbating ischemic injury, thereby raising concerns about its safety profile in this context (Warner et al., 2019