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Inflammation was induced by the stimulation of lipopolysaccharides (LPS, 100 ng/mL), which led to the generation of inflammatory mediators and oxidative stress, including ROS

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Owing to their high oxygen consumption, skeletal muscle fibers are inherently prone to ROS generation during contraction and metabolism ( Elevated ROS also activate the NF-κB pathway by depleting glutathione (GSH), downregulating myogenic transcription factors (MyoD, MyoG), and upregulating the transcriptional repressor Yin Yang 1 (YY1), thereby impairing myogenic differentiation (Xiang et al., 2022)