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Specifically, under aerobic conditions and in aqueous media (for instance, the extra‐ and intracellular media), selenoneine is expected to be oxidized to selenoneine diselenide
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These newly formed radicals in turn oxidize the spectrophotometric marker ( N,N -diethyl- p -phenylenediamine), which is detectable by absorption at 505 nm as Carratelli units, where one unit is equal to 0.8 mg hydrogen peroxide/L
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