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Mitochondria are the main participants of oxidative stress, and their proteins are observed to be oxidized damaged in the X-ALD model, leading to the dysfunction of key enzymes in mitochondrial energy metabolism, such as α/β-ATP synthase, the trichloroacetic acid cycle enzymes malate dehydrogenase, and aconitase (105)

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The best single test to screen for both conditions is the serum thyroid stimulating hormone (TSH) concentration, which is suppressed to a low level in people with hyperthyroidism, and elevated in those with hypothyroidism