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A recent study proposed that this can be achieved using a digital droplet PCR assay targeting midportion or 3′ terminal end of HBV S transcripts, the latter being absent in transcripts derived from integrated HBV DNA, but this assay relies on liver tissue and an assay that can be applied to blood samples is needed.93 The complete suppression of cccDNA transcription is best determined by quantifying intrahepatic pgRNA, but this is not practical for clinical trials
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