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For example, Thr356 in hDAT facilitates a change in hDAT to the outward-open conformation upon substrate binding and promotes the substrate efflux, however, the mutation Thr356Met associated with autism spectrum disorder blocks conformational changes in hDAT leading to effects on transport cycle (Hamilton et al., 2013)
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Once a vial passes its labeled date, the benzyl alcohol preservative can no longer be relied upon to maintain its specified concentration, undermining the foundational property that makes bacteriostatic water useful in research settings
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