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Functional studies include [ 14 C]-propionate or [ 14 C]- N 5− methyl-tetrahydrofolate incorporation into cellular macromolecules, to assess the activities of MCM and MS, respectively, and biosynthesis of MeCbl and AdoCbl upon metabolic labeling with holo-TC made from apo-TC and commercially available [ 57 Co]-vitamin B 12 (Watkins and Rosenblatt, 2013)