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Because water is amphiprotic, one water molecule can react with another to form an OH − ion and an H 3 O + ion in an autoionization process: Equation 16.4 The equilibrium constant K for this reaction can be written as follows: Equation 16.5 When pure liquid water is in equilibrium with hydronium and hydroxide ions at 25°C, the concentrations of the hydronium ion and the hydroxide ion are equal: [H 3 O + ] = [OH − ] = 1.003 × 10 −7 M