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Importantly, in Caco-2 cells expressing the PEAK1 Y724F mutant, overexpression of Src kinase was insufficient to reverse the detrimental effects of the PEAK1 mutation, as evidenced by the continued presence of decreased ZO-1 levels, lower TEER, increased tight junction distortions, and FITC-Dextran permeability (Fig

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Protein Intake Aim for 1.6–2.2 g/kg of body weight per day to maximize MPS