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16 Synaptic vesicles then fuse with the plasma membrane of neurons, releasing glutamate from the vesicles as a neurotransmitter into the synaptic cleft

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Detection of Fe 2+ The Fe 2+ was detected by BioTracker 575 Red Fe 2+ Dye (Sigma-Aldrich) and Mito- Dojindo FerroGreen (Dojindo, Japan)