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Thymosin Beta-4 could heal ligament injuries A rat study involving the local administration of Thymosin Beta-4 discovered that Thymosin Beta-4 has “potential clinical use in repairing ligaments” [14]

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Cells were harvested using radioimmunoprecipitation assay lysis buffer supplemented with phosphatase inhibitor cocktails 1 and 2 (Sigma-Aldrich)