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This mechanism is shared with Minoxidil (which is primarily a vasodilator) but achieved through a different pathway — the two are complementary rather than redundant when used together

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Alternatively, Azurin was subcloned on the same vector with the C-terminal Strep -tag II without amber stop codon, and the Azo-tag was introduced at the N-terminus of the mature polypeptide, directly downstream of the OmpA signal sequence, via QuikChange site-directed mutagenesis (Agilent, Santa Clara, CA)