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To clarify the enzyme dependency, we reintroduced either wild-type ZDHHC13 (ZDHHC13-WT) or an enzymatically inactive mutant (ZDHHC13-C456S) 38 into Zdhhc13 -knockout cells
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Cells were collected using a cell lifter, centrifuged at 14,000 g for 10 min at 4°C, and the resulting supernatant was removed to glass vials and stored at −80°C before analysis
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