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Spleen, liver, kidney and skin samples were from female animals, and muscle was from a male animal
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However, due to the lack of operable industry standards and regulation, the abuse of anti-disease drugs (Casali et al., 2005), serious pollution of aquaculture water and environmental degradation, it leads to overloading of the liver, intestines and other digestive organs of bullfrogs and abnormal lipid metabolism, which results in excessive accumulation of liver and abdominal fat, damaging the liver, gallbladder and intestinal organs, and causing diseases such as liver enlargement, hepatitis, enteritis, etc (Keum Taek et al., 2002), which seriously restricts growth and development of bullfrogs and even kills them in serious cases (Wei et al., 2023)