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Increased levels have been observed in cancer of the ovary, liver, lung, and breast, and in melanoma and leukemia (Fujisawa et al., 1976
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To generate adh-5 null mutant, we utilized a universal STOP-IN cassette that contained an exogenous Cas9 target site, multiple stop codons in all three reading frames, and the recognition site of the NheI restriction enzyme 23
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