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Instruments and equipment WQF-530 FTIR Fourier transform infrared spectrometer (Beijing Beifen Ruili Analytical Instruments (Group) Co., Ltd.), 759S UV-Vis spectrophotometer (Shanghai Prism Technology Co., Ltd.), Talos F200s high-resolution transmission electron microscope (Thermo Scientific), K-Alpha X-ray photoelectron spectrometer (Thermo Scientific), and NIRSA5.8 software developed by the NIR team of Jiangsu University for IR spectral data processing

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Cells were grown overnight in an anaerobic chamber in minimal A medium with 0.2% glucose, 0.2% casamino acids, and 0.5 mM tryptophan