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Western blot analysis Proteins were extracted from the stored lung tissue samples and A549 cell samples using radioimmunoprecipitation assay (RIPA) lysis buffer (Cell Signaling, US)

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Future research should prioritise long-term outcomes in high-risk groups, comparisons of physiological versus pharmacological dosages in healthy subjects, cost-effectiveness analyses, and adherence monitoring to inform clinical guidelines for the management and prevention of B12 deficiency