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NK cells and CD8 + Teffs are particularly sensitive even to low doses of H₂O₂, whereas Tregs exhibit stronger resistance due to distinct metabolic wiring [12, 13, 207, 208]

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The whole brain was removed and homogenized in ice-cold 0.1 M phosphate buffer (pH 7.4), then centrifuged at 12,000 rpm for 10 min at 4°C