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The distribution of the charge on surface and internal is not even, so a solution with pH value slightly different to protein pI could not insure the protein exhibit an expected charged surface
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Mark Brönstrup, Helmholtz Centre for Infection Research, Braunschweig, Germany), or palmitate 0.5 mM (Sigma) were added to the media 24 h after adeno-Cre infection in monolayer cultures
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