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The application of next-generation sequencing, including phylogenetic marker gene-based amplicon studies [e.g., bacterial 16S or fungal internal transcribed spacer (ITS) sequencing] and shotgun metagenomic sequencing, has greatly facilitated research on the human microbiota, allowing us to uncover the mysteries of our second genome, acquired after birth, and to explore the interactions of the microbiota with the host

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In addition, special care needs to be taken before any surgical procedures