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Limitations of Research Methods for Determining Enzymatic Mechanisms Underlying Alcohol Metabolism and Future Prospects Research on the enzymatic mechanisms underlying alcohol metabolism has been carried out by examining the relationships between the biochemical and enzymatic properties of each alcohol oxidase (e.g., Kms for EtOH, inhibitor specificities, activity changes under various conditions) and the rates of alcohol metabolism measured at various levels (isolated hepatocytes, hepatic perfusion, and individually in vivo)
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