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Transfection and development of stable fibroblast cells containing VEGF 165 1 × 10 5 Hu02, human foreskin fibroblast cells that were purchased from the Iranian Biological Resource Center, cultured in 24-well plates one day before transfection in dulbecco’s modified eagle medium (DMEM)/F-12 medium supplemented with 2 mM L-glutamine and 10% fetal bovine serum (FBS) (Gibco, USA)